Journal: Journal of Virology

Article Title: Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model

doi: 10.1128/jvi.00556-23

Figure Lengend Snippet: ZIKV infection of the brain elicits proinflammatory responses in Ifnar1 −/− mice. (A) Ifnar1 −/− mice were subcutaneously infected with 10 3 PFU of ZIKV or PBS (mock). They were monitored daily for a neurological disease score as described in Materials and Methods. (B) Ifnar1 −/− mice ( n = 5 for males and n = 3 for females) were infected with 10 3 PFU of ZIKV. Viral RNA levels in the brain, spinal cord, eyes, kidney, testes, and ovary were assessed using RT-qPCR at 6 dpi. (C) RNA extracted from the brain and spinal cord homogenates was used to assess mRNA levels of proinflammatory cytokines, including IL-1β, IL-6, IFN-γ, and TNF-α, by RT-qPCR at 6 dpi. (D) The brain homogenates were used to measure protein levels of proinflammatory cytokines by ELISA at 6 dpi. Statistically significant differences between the groups were determined using multiple two-tailed t tests (A), one-way analysis of variance (ANOVA; B), and Student t test (C and D). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Bars indicate means ± the standard errors of the mean (SEM).

Article Snippet: Isolated brain mononuclear cells from the mock- or ZIKV-infected mice in cell staining buffer (PBS with 1% FBS and 0.09% NaN 3 ) were stained for 30 min with fluorescence-conjugated antibodies, namely, Brilliant Violet 421 anti-mouse/human CD11b antibody (101236; BioLegend, San Diego, CA), PE/Cyanine7 anti-mouse CD45 Antibody (103114; BioLegend), APC anti-mouse TNF-α antibody (506307; BioLegend), FITC anti-mouse IL-6 monoclonal antibody (MP5-20F3; 11-7061-82; eBioscience, San Diego, CA), PE anti-mouse IL-1β antibody (12-7114-82, eBioscience), and Alexa Fluor 488 anti-flavivirus group antigen antibody (4G2; NBP2-52709AF488; Novus Biologicals, Englewood, CO).

Techniques: Infection, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: Journal of Virology

Article Title: Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model

doi: 10.1128/jvi.00556-23

Figure Lengend Snippet: Proinflammatory activation of macrophages by ZIKV infection in the mouse brain. (A) Representative flow plots gated on microglia (upper) and macrophages (lower) to show expression levels of proinflammatory cytokines, including IL-1β, IL-6, and TNF-α. (B and C) Bar graphs indicate the percentage of activated microglia (B) and macrophages (C) expressing IL-1β (left), IL-6 (middle), and TNF-α (right). Statistically significant differences between the groups were determined using Student t test (B and C). ns, not significant. **, P < 0.01; ****, P < 0.0001. Bars indicate means ± the SEM.

Article Snippet: Isolated brain mononuclear cells from the mock- or ZIKV-infected mice in cell staining buffer (PBS with 1% FBS and 0.09% NaN 3 ) were stained for 30 min with fluorescence-conjugated antibodies, namely, Brilliant Violet 421 anti-mouse/human CD11b antibody (101236; BioLegend, San Diego, CA), PE/Cyanine7 anti-mouse CD45 Antibody (103114; BioLegend), APC anti-mouse TNF-α antibody (506307; BioLegend), FITC anti-mouse IL-6 monoclonal antibody (MP5-20F3; 11-7061-82; eBioscience, San Diego, CA), PE anti-mouse IL-1β antibody (12-7114-82, eBioscience), and Alexa Fluor 488 anti-flavivirus group antigen antibody (4G2; NBP2-52709AF488; Novus Biologicals, Englewood, CO).

Techniques: Activation Assay, Infection, Expressing

Journal: iScience

Article Title: Mannose-coated nanozyme for relief from chemotherapy-induced peripheral neuropathic pain

doi: 10.1016/j.isci.2023.106414

Figure Lengend Snippet: Key resources table

Article Snippet: APC anti-mouse IL-6 antibody , BioLegend, USA , Cat# 504507; RRID: AB_10694094.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software, Transmission Assay, Electron Microscopy, Fourier Transform Infrared Spectroscopy, Spectroscopy, X-ray Diffraction, Electron Paramagnetic Resonance, Confocal Laser Scanning Microscopy, Modification